Figure 1. Triiodothyronine (T₃)-treated mice show classic effects of high thyroid hormone levels compared to control mice (CON).

(A–F) Serum levels of T₃ and thyroxine (T₄), 24 hr profiles of locomotor activity, body temperature, O₂ consumption, and respiratory quotient are shown. Rhythm evaluation was performed by JTK_CYCLE (p<0.01, Supplementary file 1). Presence (R) or absence of circadian rhythm (NR) is depicted. In the presence of significant 24 hr rhythmicity, a sine curve was fit. In (A) and (B), data are double plotted to emphasize the absence or presence of rhythms. (G–I) Linear regression of T₃ average levels with average of locomotor activity, temperature, and O₂ consumption. (J–O) Correlation between thyroid hormone levels and normalized levels of metabolic outputs is shown as z-scores (additional information is described in Supplementary file 2). In (A) and (B), n = 4–6 animals per group and/or timepoint. In (C) and (D), n = 4 and 5 for CON and T₃ groups, respectively. In (E) and (F), n = 4 for each group.

Figure 1—figure supplement 1. Metabolic evaluation of control (CON) and triiodothyronine (T₃) mice.

(A–D) Assessment of body temperature, food and water intake (per cage, n = 8), and body weight. (E–I) Metabolic parameters (described in the y-axis) were obtained from the third week of experiment (days 19/20). Day and night data were obtained by averaging values from Zeitgeber time (ZT) 0–12 (day) and from ZT 12–24 (night) and plot accordingly. Letters represent a difference between the same group in day vs. night comparisons. Asterisks represent significant differences between CON and T₃ mice. In (H), 95% confidence intervals are shown. Comparison of the slope and elevations/intercept between the groups was performed: p=0.30 and 0.01, respectively. Data are shown either as mean ± SEM or by boxplot. n = 24 for (A) and (D). (E–I) n = 4–5 per group.