Fig. 4.

Rbm47 knockdown compromises the multi-lineage differentiation potential of mESCs. A An image of the teratomas harvested from indicated NOD/SCID mice groups (Number of mice in each group: shlacZ = 6; shRbm47#1 = 6 and shRbm47#3 = 6). B Mean teratoma volumes were calculated for each group and plotted as mean ± S.D. C Representative images of HE-stained sections of teratomas from control and Rbm47 knockdown mESCs displaying structures from three lineages: Mesoderm- osteoid/primitive bone-like (i); Ectoderm- primitive neuroepithelium (ii), a squamous epithelium with keratin pearls (iv); Endoderm- columnar epithelium/secretory glands (iii & v). Scale bar, 20 μm. D RT-qPCR profiling of control and Rbm47 knockdown mESCs differentiated for 6-days as a monolayer culture in a serum-supplemented medium. Mean log2 relative mRNA expression ± S.E.M values were plotted from three biological replicates. Statistical test used for B- unpaired student’s t-test; D- ordinary one-way ANOVA followed by Dunnett’s multiple comparison test; ns- non-significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001