TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Strain | ||
| Legionella pneumo phila | ||
| AM511 | Philadelphia 1 r− m− Smr | 13 |
| CS332 | Philadelphia 1 LPS mutant Smr | This study |
| CS333 | AM511 harboring pLAW300 | This study |
| CS334 | CS332 harboring pLPS16 | This study |
| CS338 | CS332 harboring pLPS17 | This study |
| CS339 | CS332 harboring pLPS20 | This study |
| Escherichia coli | ||
| DH5α | Nalr r− m+ | C. Collins |
| Plasmid | ||
| pAM2 | IncP cosmid Gmr Tcr mob+ | 13 |
| pRK2073 | ColE1 IncP tra+ | D. Figurski |
| pUC19 | cloning vector ColE1 Apr | New England Biolabs |
| pGEM-7Zf(−) | cloning vector, Apr | Promega |
| pLAW300 | pBlueScript II SK +/− with 939-bp HindIII fragment from Tn903 ColE1 Kmr | H. Shuman |
| pLOI193 | IncQ cloning vector, Cmr Tcr | C. Eddy |
| pLPS16 | 4.5-kb SphI fragment from pLPS15 cloned into pLOI193 | This study |
| pLPS16.1 | 4.5-kb SphI fragment from pLPS15 cloned into pUC19 | This study |
| pLPS17 | 2.3-kb EcoRI fragment from pLPS16.1 cloned into pLAW300 | This study |
| pLPS20 | 1.2-kb EcoRI fragment from pLPS16.1 cloned into pLAW300 | This study |
a
Apr, ampicillin resistant; Cmr, chloramphenicol resistant; Gmr, gentamicin resistant; Kmr, kanamycin resistant; Nalr, naladixic acid resistant; Smr, streptomycin resistant; Spr, spectinomycin resistant; Tcr, tetracycline resistant; r−, restriction minus; m+, modification plus; m−, modification minus; mob+, mobilization plus; tra+, transfer plus.