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. 2022 Jan 26;18(6):2028–2044. doi: 10.1007/s12015-021-10288-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Differentiation Assays of moMSCORS. Differentiation capacities of moMSCORS. moMSCORS exhibited differentiation potentials towards 5 lineages including Chondrogenic, Adipogenic, Osteogenic, endothelial cells and smooth muscle cells. (A) Chondrogenic differentiation for 21 days using pellet culture, and the production of proteoglycan was detected using Alcian Blue staining. (B) Adipogenic differentiation for 21 days, the lipid vesicles were detected using Oil Red O staining. (C,D) Osteogenic differentiation of moMSCORS after 21 days induction. The calcium deposition was detected using Alizarin Red staining, and the activity of alkaline phosphatase visualized by the BCIP/NBT assay. (E,F) Endothelial differentiation of moMSCORS for 21 days was verified using CD31 immunostaining, and the vascular anastomosis was detected using the tube forming assay with Live/Dead assay (Calcein AM/Propodium Iodide). (G) Smooth muscle cell differentiation for 21 days, which was detected using αSMA immunostaining. Scale bar corresponds to (A,B,G) 100 μm, (C,D,F) 500 μm, (E) 200 μm; magnification (A,B,G) 20x, (C,D,F) 4x, (E) 10x