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. 2022 Feb 21;18(6):2164–2178. doi: 10.1007/s12015-022-10350-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Mouse BMSCs proliferation, migration, morphology, and fusion to human myoblasts. (A) Scratch wound healing assay 6h and 24h after performing the scratch for control or transfected with miR151 or miR5100 BMSCs. (B) Proliferation (CFSE assay) of control or miR151 or miR5100 transfected (24h or 48h) mouse BMSCs. (C) Giemsa staining of control and miR151 or miR5100 transfected mouse BMSCs. (D) Fusion index of human myoblasts cultured in the presence of control or miR151 or miR5100 transfected mouse BMSCs, (E) Visualization of skeletal myosin (red), human nuclear antigen (green) and cell nuclei (blue) in cocultures of myoblasts with control or miR151 or miR5100 transfected BMSCs. Mouse nuclei (blue) within myotubes marked with arrows. Scale: 20 μm. The differences were considered statistically significant when p < 0.05 (marked with asterisks, * - p < 0.05; ** - p < 0.005; *** - p < 0.001)