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. Author manuscript; available in PMC: 2023 Aug 18.
Published in final edited form as: Cell Chem Biol. 2022 May 31;29(8):1333–1340.e5. doi: 10.1016/j.chembiol.2022.05.003

Figure 2.

Figure 2.

TR-FRET-based quantification of BRD4 levels in unmodified cell lines after degrader treatment. BRD4 protein levels in cell lysate after 5 h treatment with dBET6 or JQ1 were measured with TR-FRET assay as described in Figure 1A. Assays were run in a 24-well plate format with either (A) MCF7 or (B) MDA-MB-231 cells. Western blot analyses on the same samples are shown in the bottom panels. (C-D) Quantitative profiling of BRD4 degradation rescue by co-treatment of (C) MCF7 or (D) MDA-MB-231 cells with dBET6 and BTZ, MLN7243, MLN4924, JQ1, or POM after 5 h shows efficient attenuation of degradation. Data in (A-D) are expressed as mean ± SD of 2 technical × 2 biological replicates. Unprocessed Western blot data is shown in Figure S4.