KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
|
| ||
| MCT1/SLC16A1 | Cell signaling | Cat# 85680 |
| ATP-Citrate Lyase | Cell signaling | Cat# 4332 |
| PCK1 (D12F5) | Cell signaling | Cat# 12940 |
| Acetyl-Histone H3 (Lys9) (C5B11) | Cell signaling | Cat# 9649 |
| Acetyl-Histone H3 (Lys27) | Cell signaling | Cat# 4353 |
| Histone H3 (1B1B2) | Cell signaling | Cat# 14269 |
| PDHA1 | Invitrogen | Cat# 8D10E6 |
| Acetyl-Histone H3 | Sigma | Cat# 06-599 |
| Histone H3K14ac | Active Motif | Cat# 61433 |
| PCB | Abcam | Cat# ab115579 |
| 14-3-3 (C20) | Santa Cruz | Cat# sc-628 |
| V5-Tag (E9H8O) | Cell signaling | Cat# 80076 |
| β-actin | Sigma | Cat# A1978, clone AC15 |
| Flag | Sigma | Cat# F3165 |
| anti-rabbit IgG (H + L) secondary antibody, HRP | Thermo Fisher | Cat# 31460 |
| anti-mouse IgG (H + L) secondary antibody, HRP | Thermo Fisher | Cat# 31430 |
|
| ||
| Chemicals, Peptides, and Recombinant Proteins | ||
|
| ||
| BMS-303141 | MedChemExpress | Cat# HY-16107 |
| Oligomycin A | Selleckchem | Cat# S1478 |
| IACS-010759 | Selleckchem | Cat# S8731 |
| Devimistat (CPI-613) | Selleckchem | Cat# S2776 |
| Pronase | Sigma-Aldrich | Cat# 10165921001 |
| Sodium Acetate | Sigma-Aldrich | Cat# S2889 |
| L-(+)-Lactic acid >98% | Sigma-Aldrich | Cat# L1750 |
| Sodium L-lactate | Sigma-Aldrich | Cat# L7022 |
| D-Glucose (U-13C6, 99%) | Cambridge Isotope Laboratories, Inc | Cat# CLM-1396-2 |
| Sodium L-Lactate (3-13C, 98%) 20% w/w in H2O | Cambridge Isotope Laboratories, Inc | Cat# CLM-1578-PK |
| Sodium L-Lactate (Microbiological/Pyrogen Tested) (13C3, 98%) 20% w/w in H2O | Cambridge Isotope Laboratories, Inc | Cat# CLM-1579-MPT-PK |
|
| ||
| Critical Commercial Assays | ||
|
| ||
| NuPAGE™ 4 to 12%, Bis-Tris, 1.0–1.5 mm, Mini Protein Gels | Thermo Fisher | Cat# NP0321BOX |
| CellTiter-Glo® Luminescent Cell Viability Assay | Promega | Cat# G7571 |
| Crystal Violet Assay Kit | Abcam | Cat# ab232855 |
| 16% Formaldehyde (w/v), Methanol-free | Thermo Fisher | Cat# 28908 |
| Lipofectamine RNAiMAX Transfection Reagent | Invitrogen | Cat# 13778075 |
| QuikChange II XL Site-Directed Mutagenesis Kit | Agilent | Cat# 200521 |
| Seahorse XFp Glycolysis Stress Test Kit | Agilent | Cat# 103017-100 |
| Seahorse XFp Cell Mito Stress Test Kit | Agilent | Cat# 103010-100 |
| Annexin V Apoptosis Detection Kit | BD Biosciences | Cat# 556547 |
| Propidium Iodide (PI)/RNase Staining Solution | Cell signaling | Cat# 4087S |
| Click-iT EdU Alexa Flour 488 Flow Cytometry Assay Kit | Thermo Fisher | Cat# C10632 |
| miRNeasy Mini kit | Qiagen | Cat# 217004 |
| First Strand cDNA Synthesis Kit | Origene | Cat# NP100042 |
| PerfeCta® SYBR® Green FastMix | VWR | Cat# 101414-270 |
| SimpleChIP® Enzymatic Chromatin IP Kit | Cell signaling | Cat# 9003 |
| ATAC-Seq Kit | Active Motif | Cat# 53150 |
|
| ||
| Deposited Data | ||
|
| ||
| Microarray data | GEO database | ID: GSE145699 |
| ChIP-seq data | GEO database | ID: GSE151852 |
| ATAC-seq data | GEO database | ID: GSE162831 |
| Raw Data Western Blot | Mendeley | http://dx.doi.org/10.17632/pd854w5cyy.1 |
|
| ||
| Experimental Models: Cell Lines | ||
|
| ||
| KNS42 | Japanese Collection of Research Bioresources Cell Bank | Cat# IFO50356 |
| U87-MG | American Type Culture Collection | Cat# HTB-14 |
| DBRTG-05MG | American Type Culture Collection | Cat# CRL-2020 |
| U251 | Sigma | Cat# CB_09063001 |
| NCH644 | Cell Line Services | Cat# 300124 |
| IDH1(R132H) U87 | American Type Culture Collection | Cat# HTB-14IG |
| U87-EGFRVIII | Ludwig Institute for Cancer Research | Frank Furnari |
| GBM22 cells | Mayo Clinic | Jann Sarkaria |
| GBM43 cells | Mayo Clinic | Jann Sarkaria |
| GBM12 cells | Mayo Clinic | Jann Sarkaria |
| Astrocyte | ScienceCell Research Laboratories | Cat# 1800 |
|
| ||
| Oligonucleotides | ||
|
| ||
| ON-TARGETplus Non-targeting Pool | Dharmacon | Cat# D-001810-10 |
| ON-TARGETplus Human SLC16A1siRNA#6 | Dharmacon | Cat# J-007402-06 |
| ON-TARGETplus Human SLC16A1siRNA#7 | Dharmacon | Cat# J-007402-07 |
| ON-TARGETplus Human SLC16A1siRNA#8 | Dharmacon | Cat# J-070402-08 |
| ON-TARGETplus Human PDHA1 siRNA | Dharmacon | Cat# L-010329-00-0005 |
| ON-TARGETplus Human PC siRNA | Dharmacon | Cat# L008950-00-0005 |
| ON-TARGETplus Human ACLY siRNA#1 | Dharmacon | Cat# J-004915-05 |
| ON-TARGETplus Human ACLY siRNA#2 | Dharmacon | Cat# J-004915-06 |
| ON-TARGETplus Human ACLY siRNA#3 | Dharmacon | Cat# J-004915-07 |
| ON-TARGETplus Human ACLY siRNA#4 | Dharmacon | Cat# J-004915-08 |
| shSLC16A1#35 | Sigma | Cat# TRCN0000038535 |
| shSLC16A1#39 | Sigma | Cat# TRCN0000038339 |
| shSLC16A1#40 | Sigma | Cat# TRCN0000038340 |
| shPDHA1#582 | Sigma | Cat# TRCN0000028582 |
| shPDHA1#590 | Sigma | Cat# TRCN0000028590 |
| shPDHA1#602 | Sigma | Cat# TRCN0000028602 |
| shACLY#3 | Sigma | Cat# TRCN0000078283 |
| shACLY#4 | Sigma | Cat# TRCN0000078284 |
|
| ||
| Recombinant DNA | ||
|
| ||
| pLV-EF1-NDI1-IRES-RFP-WPRE | Northwestern University | Navdeep Chandel |
| pLV-EF1-ORF-IRES-RFP-WPRE | Northwestern University | Navdeep Chandel |
| pCDH-3xFlag | Wake Forest School of Medicine, Winston-Salem, NC | Hui-Kuan Lin |
| pCDH-PDHA-WT-Flag-Blasticidin | Wake Forest School of Medicine, Winston-Salem, NC | Hui-Kuan Lin |
| pLX304 | Genecopoeia | EX-NEG-LX304 |
| pLX304_ACLY_V5 | Genecopoeia | EX-OL00009-LX304 |
|
| ||
| Software and Algorithms | ||
|
| ||
| Galaxy software | https://usegalaxy.org/ | N/A |
| Integrated Genome Browser 9.1.8 | BioViz.org. | N/A |
| GraphPad Prism 9 | GraphPad | N/A |
| MataboAnalysis 5.0 | https://www.metaboanalyst.ca/home.xhtml | N/A |
| Basepair | Basepair Tech | N/A |
| CompuSyn | ComboSyn, Inc. | N/A |
| FlowJo software (version 10.8.1) | BD | N/A |
| Azure (C300) imaging system | Azure Biosystems | N/A |