FIG. 5.

Alignment of N-terminal sequences of the M. voltae FlaB2 flagellin, the P. aeruginosa PilA pilin, and the products of the two fusion constructs with their leader peptides intact. The position of leader peptide cleavage shown represents that known for the M. voltae and P. aeruginosa proteins and predicted for the fusions. Shown in boldface are amino acid residues of interest in the fusion proteins: the +1 position change of the mature M. voltae FlaB2 to a phenylalanine from an alanine in both fusions, and the change in the +5 position of the mature FlaB2 from an glycine to a glutamic acid in the fusion encoded by pKJ248.