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. Author manuscript; available in PMC: 2023 Jan 6.
Published in final edited form as: Mucosal Immunol. 2022 Jul 6;15(5):837–845. doi: 10.1038/s41385-022-00541-8

Figure 1: Pros and cons of multi -omic approaches applied to lower airway studies.

Figure 1:

This figure summarizes the challenges encountered with different omic technologies that are currently in use for lower airway microbiome studies. Here we make an illustration of both sequencing-based bioinformatic tools (16S ribosomal RNA (rRNA) gene amplicon sequencing, 18S rRNA gene amplicon sequencing, metagenomics, metatranscriptomics and host transcriptomics) and mass spectrometry-based tools (metabolomics and proteomics); highlighting technology-specific pros and cons. On the right in the figure, we show several key challenges that are commonly shared in varying degrees between the different omic approaches when studying the lower airways.