Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and virus strains | ||
| E. coli strain BL21 (DE3) Rosetta2 | Millipore | Cat#71400 |
| DH5-alpha electrocompetent E. coli cells | Goldbio | Cat#CC-203-TR |
| Biological samples | ||
| Chemicals, peptides, and recombinant proteins | ||
| EB-47 (hydrochloride) | Cayman Chemical | Cat#34684 |
| Talazoparib | SelleckChem | Cat#S7048 |
| Veliparib (ABT-888, hydrochloride) | Cayman Chemical | Cat#11505 |
| Hydroxylamine (NH2OH) | Sigma-Aldrich | Cat#467804 |
| Critical commercial assays | ||
| Lipofectamine 3000 | Thermofisher | Cat#L3000001 |
| Deposited data | ||
| PARP1 ΔART bound to a 10-bp palindromic DNA duplex | This paper | PDB: 7S68 |
| PARP1 ΔVE bound to EB-47 and a 5-bp DNA duplex | This paper | PDB: 7S6H |
| PARP1 ΔVE bound to a 5-bp DNA duplex | This paper | PDB: 7S6M |
| PARP1 ΔART bound to a 12-bp palindromic DNA duplex | This paper | PDB: 7S81 |
| Structure of Human PARP-1 bound to a DNA double strand break | (Langelier et al., 2012) | PDB: 4DQY |
| Crystal structure of the catalytic domain of human PARP1 (apo) | (Ogden et al., 2021) | PDB: 7AAA |
| Mendeley Data (uncropped gel images) http://dx.doi.org/10.17632/cx5dcgnmtp.1 | This paper | http://dx.doi.org/10.17632/cx5dcgnmtp.1 |
| Experimental models: Cell lines | ||
| CAL51 PARP1−/− | (Pettitt et al., 2018) | N/A |
| Experimental models: Organisms/strains | ||
| Oligonucleotides | ||
| 10 nt palindromic DNA | IDT | N/A |
| 12 nt palindromic DNA | IDT | N/A |
| 5 nt DNA | IDT | N/A |
| 48 nt DNA carrying an internal 6-FAM group (dumbbell) | IDT | N/A |
| 48 nt DNA (dumbbell) | IDT | N/A |
| 18 nt DNA with a 6-FAM group on the 5’ end | IDT | N/A |
| Recombinant DNA | ||
| PARP1 ΔART | This paper | N/A |
| PARP1 L698A/L701A | (Langelier et al., 2012) | N/A |
| PARP1 G558E | This paper | N/A |
| PARP1 ΔV687-E688 | (Pettitt et al., 2018) | N/A |
| PARP1 M686G/V687P | This paper | N/A |
| PARP1 V687A/E688A | This paper | N/A |
| PARP1 V687A | This paper | N/A |
| PARP1 E688A | This paper | N/A |
| PARP1 L713F | (Langelier et al., 2012) | N/A |
| PARP1 Y569A | (Langelier et al., 2012) | N/A |
| PARP1 Y569L | This paper | N/A |
| PARP1 G558L | This paper | N/A |
| PARP1 G558V | This paper | N/A |
| PARP1 Zn1-Zn3 ΔZn2 | (Steffen et al., 2015) | N/A |
| PARP1 Zn1 | (Langelier et al., 2010) | N/A |
| PARP1 Zn3 | (Langelier et al., 2008) | N/A |
| PARP1 WGR-CAT | (Langelier et al., 2012) | N/A |
| PARP1 WGR-HD | This paper | N/A |
| PARP1-GFP | (Pettitt et al., 2018) | N/A |
| Software and algorithms | ||
| XDS | (Kabsch, 2010) | https://xds.mr.mpg.de |
| PHASER | (McCoy et al., 2007) | |
| CCP4 | (Winn et al., 2011) | https://www.ccp4.ac.uk |
| COOT | (Emsley et al., 2010) | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| REFMAC5 (CCP4 suite) | (Murshudov et al., 2011) | https://www.ccp4.ac.uk/html/refmac5.html |
| Phenix | (Liebschner et al., 2019) | http://www.phenix-online.org |
| phenix.refine (Phenix suite) | (Afonine et al., 2012) | https://phenix-online.org/documentation/reference/refinement.html |
| SBGrid | (Morin et al., 2013) | https://sbgrid.org |
| Matlab | MathWorks | https://www.mathworks.com/products/matlab.htm |
| TraceDrawer | Ridgeview Instruments | https://tracedrawer.com |