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DNA hybridization analysis of pilA locus structure for S- and L-phase variants of E. corrodens VA1. Total DNA (4 μg per lane) was isolated from strain VA1-S1 (lanes 1) or VA1-L2 (lanes 2), digested with PvuII and BglII (A), PvuII (B), or PvuII and DraI (C), and subjected to blot hybridization against a DNA probe for the pilA locus of strain VA1-S1. The positions of DNA molecular size standards are shown at the left.
