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Localization of PilA1 in S- and L-phase variants of E. corrodens VA1. Surface (lanes 1 and 2), soluble (lanes 3 and 4), and insoluble (lanes 5 and 6) protein fractions from strains VA1-S1 (lanes 1, 3, and 5) and VA1-L2 (lanes 2, 4, and 6) were subjected to immunoblot analysis with a polyclonal antiserum specific for PilA1. The arrow marks the position of mature pilin. The positions of protein molecular weight standards are shown at the left.
