TABLE 1.
Strains and plasmids used
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| E. corrodens | ||
| VA1 | Clinical isolate | Laboratory collection |
| VA1-S1, -S2, and -S3 | S-phase variants of VA1 | Laboratory collection |
| VA1-L2 | L-phase variant of VA1 | Laboratory collection |
| E. coli | ||
| DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) supE44 λ− thi-1 gyrA96 relA1 | Bethesda Research Laboratories |
| BL21(DE3) | F−ompT hsdSB(rB− mB−) gal dcm (DE3) | Novagen |
| Plasmids | ||
| pGEM3zf(+) | Apr cloning and sequencing vector | Promega |
| pET-22b | Apr expression vector | Novagen |
| pVD203 | Carries pilE1 gene from N. gonorrhoeae | 7 |
| pEC114 | pGEM3zf(+) with 3.9-kbp EcoRI fragment from VA1-S1 containing pilA locus | This study |
| pEC203 | pBluescript SK (+) with 0.6-kbp EcoRI fragment from VA1-S1 containing pilA1 sequences, used to generate pilA1-specific RNA probes | This study |
| pEC205 | pGEM3zf(+) with 0.9-kbp PvuII fragment from VA1-S1 containing mostly pilA2 sequences, used to generate pilA2-specific RNA probes | This study |
| pEC206 | pET-22b with DNA fragment from VA1-S1 encoding residues 26–146 of PilA1 | This study |