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. 2022 Aug 19;12:14162. doi: 10.1038/s41598-022-18422-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Hierarchical clustering heatmaps of RNA-seq expression for genes potentially involved in the inflammatory chemokine and citokine (a), PDGF (b) and GnRHR (c) signaling pathways. The maps were generated with the ‘pheatmap’ R package (https://CRAN.R-project.org/package=pheatmap). (d) qRT-PCR validation of the changes in expression of selected genes classified into these pathways. Data from qRT-PCR are the mean ± SEM (n = 3 pools of 3 different fish each) and were plotted using the GraphPad Prism v8.4.3 (686) software (https://www.graphpad.com/).