Table 1.
Top 10 upregulated mRNAs in SPZEJ, their fold-change in expression with respect to HGC and mean of fragments per kilo base of transcript per million mapped fragments (FPKM), and their potential associated functions according to GeneCards (www.genecards.org).
| Product | Gene | Sequence ID | Log2 FC | Mean FPKM | Reproductive-related function |
|---|---|---|---|---|---|
| Toll-like receptor 1 | tlr1 | XM_030396315.1 | 11.16 | 1698 | Involved in ovulation, sperm capacitation and fertilisation |
| Zona pellucida protein X, partial | zp | AAY21008.1 | 11.02 | 657 | Sperm-egg interaction |
| Transcription factor MafB | mafb | XP_030276052.1 | 10.69 | 558 | Acts as a transcriptional activator or repressor that may be involved in spermatogenesis. Unknown function in spermatozoa |
| Amyloid beta A4 precursor -binding family B member 1 | apbb1 | XP_030254479.1 | 10.08 | 236 | Transcription coregulator that can have both coactivator and corepressor functions. Can bind modified histones and chromatin modifying enzymes, thereby regulating transcription |
| CD209 antigen C | cd209c | XP_030265713.1 | 10.05 | 839 | C-type lectin that functions in cell adhesion and pathogen recognition. Unknown function in spermatozoa |
| Four and a half LIM domain 2 | fhl2 | XP_030299463.1 | 10.03 | 226 | May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Unknown function in spermatozoa |
| H-2 class II histocompatibility antigen, A-Q alpha chain | h2-aa | XP_030263565.1 | 9.96 | 1644 | Binding and presentation of peptides derived from antigens. Unknown function in spermatozoa |
| Tyrosine-protein kinase receptor TYRO3 | tyro3 | XP_030287857.1 | 9.88 | 308 | Involved in spermatogenesis. Unknown function in spermatozoa |
| Arachidonate 15-lipoxygenase B | alox15b | XP_030274670.1 | 9.83 | 212 | Catalyzes the peroxidation of free and esterified polyunsaturated fatty acids (PUFAs) generating a spectrum of bioactive lipid mediators. Involved in the oxidative stress cascade of spermatozoa |
| Inactive Rho GTPase-activating protein 11B | arhgap11b | XP_030261033.1 | 9.83 | 647 | Inhibits the mitochondrial permeability transition pore (mPTP), thereby increasing mitochondrial Ca2+. Unknown function in spermatozoa |