Figure 1.

Capillary based method for measuring basal salivation in mice. (A) Phenol red-coated thread is inserted into a fire-polished glass capillary with 3 mm extending outside the opening. This is then placed under the tongue of an isoflurane anesthetized mouse for 10 s. (B) Saliva travels up the thread, discoloring it. (C) The distance traveled in mm is taken as a measure of basal saliva. (D) Sample baseline data for WT males and females shows that basal levels are similar (n = 14 per condition).