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. 2022 Aug 19;12:14182. doi: 10.1038/s41598-022-17987-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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CB1 protein is expressed in a subset of cholinergic axons in submandibular gland. (A,B) Left panel shows CB1 (green) and cadherin (red) in wild type (WT) mouse submandibular gland. Right panel shows that CB1 staining in axon-like processes is absent submandibular gland from CB1 knockout mouse. (C) Double staining in submandibular gland of the mouse shows expression of CB1 (green) and choline acetyl transferase (ChAT, red), a marker for cholinergic axonal inputs. (D,E) CB1 (green) staining is seen in processes of ChAT-positive axons (arrows). Scale bar: (A,B) 25 µm; (C–E) 40 µm. Images processed using Adobe Photoshop vsn. 21.2 and FIJI (vsn 2.3.0/1.53q, available at https://imagej.net/Fiji/downloads).