FIG. 4.

Comparative locations of mapped genes on ApaI (top)- and NotI (bottom)-restricted genomes of the 12 A. pleuropneumoniae serotype reference strains. The top gel was run with linear ramped switch times from 5 to 20 s for 13 h and 35 to 70 s for 13 h. The bottom gel was run with switch times from 15 to 30 s for 13 h and 40 to 90 s for 17 h. The A. pleuropneumoniae serotype reference strains (S1 to S12) are grouped based on their phylogenetic relationship (7). Bacteriophage lambda concatemers were used as molecular size markers (M); the unboxed numbers indicate sizes of the corresponding bands in kilobase pairs; the boxed numbers indicate positions of the mapped genes: 1 (apxICA), 2 (apxIBD), 3 (apxIICA), 4 (apxIIICA), 5 (omlA), 6 (ureC), 7 (tolQ-tbpBA), 8 (sodA), 9 (glyA), 10 (metJ), 11 (recA), 12 (rho), and 13 (repeat element).