Figure 2.

iPSC lines with OSKM+Mettl14 OE exhibit pluripotency

(A) Morphology of the iPSCs with OSKM+Mettl14 OE lines. Scale bars, 200 μm.

(B) qRT-PCR analysis showing pluripotent gene expression in the iPSCs with OSKM+Mettl14 OE/KD relative to their expression in MEFs and ESCs. The data are presented as the means ± SEM (n = 3); ^∗p < 0.05, ^∗∗p < 0.01 by Student’s t test performed for comparison.

(C) Immunostaining analyses for the expression of pluripotent marker genes NANOG (purple), SSEA1 (purple), and OCT4 (red) and in the iPSCs with OSKM+Mettl14 OE lines. Nuclear staining by DAPI (blue). Scale bars, 25 μm.

(D) Differentiation of the embryoid bodies of the iPSCs with OSKM+Mettl14 OE line showing the differentiation potential. Scale bars, 200 μm.

(E) Hematoxylin and eosin (H&E) staining of teratomas generated by the iPSCs with OSKM+Mettl14 OE. Scale bars, 100 μm.

(F) Representative photos showing the contribution and spatial distribution of Oct4-GFP⁺ cells in the gonads of the iPSCs with OSKM+Mettl14 OE-derived chimeric embryos on embryonic day 12.5 (E12.5). Scale bars, 1 mm.