Figure 3.

The expression level of SASP genes was increased after OE of Mettl14

(A) Volcano plot showing the DEGs representing genetic changes caused by Mettl14 OE on day 15 of reprogramming.

(B) Gene Ontology (GO) enrichment analysis showing that the DEGs increased by Mettl14 were mainly enriched in the immune response.

(C) Heatmap showing SASP gene clustering in the samples on reprogramming day 15.

(D) qRT-PCR was performed to determine the expression levels of SASP genes in wild-type and mutant Mettl14 cells from days 3 to 18 and in iPSCs. The data are presented as the means ± SEM (n = 3); ^∗p < 0.05, ^∗∗p < 0.01 by Student’s t test performed for comparison.

(E) ELISAs showing the expression levels of IL-6, CXCL2, and CXCL1 in the cell-conditioned medium on reprogramming day 15. The data are presented as the means ± SEM (n = 3); ^∗p < 0.05, ^∗∗p < 0.01 by Student’s t test performed for comparison.