hPSC-derived MHE exhibits uniform hindgut patterning
hPSCs: H1, iPSC72_3, iPSC75_1, and iPSC285_1 were differentiated to day 7 MHE and immunostained with antibodies to the MHE marker CDX2 (green). Cells were counterstained with DAPI (blue). Undifferentiated H1 hESCs served as a CDX2− control. Center panel: Representative tiled 4× images of CDX2 immunostaining in 4 randomly selected adjacent fields (scale bar, 1 mm). Right panel: representative 20× images of CDX2 immunostaining (scale bars, 100 μm).