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. 2022 Jul 28;17(8):1889–1902. doi: 10.1016/j.stemcr.2022.06.011

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Agg-spheroids maintain intestinal marker expression and mesenchyme expansion

(A) H1-derived day 7 spont-spheroids and day 8 agg-spheroids were harvested, fixed, and subjected to whole-mount immunostaining for intestinal epithelial (CDX2 and CDH1) and mesenchymal (FOXF1) markers. Scale bar, 100 μm.

(B) Representative images of the gross morphology of H1-derived spont- and agg-spheroids 3 days after embedding in Matrigel. Scale bar, 100 μm.

(C) H1-derived spont- and agg-spheroids were fixed after 3 days of culture in Matrigel and subjected to whole-mount immunostaining to identify intestinal epithelia (CDH1/CDX2) and mesenchymal (FOXF1) cells. Scale bar, 100 μm.