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. 2022 Jul 28;17(8):1889–1902. doi: 10.1016/j.stemcr.2022.06.011

Figure 5.

Figure 5

Growth and cytodifferentiation of agg-HIOs is similar to spont-HIOs

(A) Gross morphology of organoids produced from H1-derived spont-spheroids (spont-HIOs) and agg-spheroids (agg-HIOs) is similar on day 35. Organoids consisted of convoluted epithelial layers (yellow arrow) surrounded by an outgrowth of mesenchymal cells (white arrow).

(B) Sections of day 35 HIOs were immunostained with antibodies to CDH1 (epithelia) and Emilin1 (mesenchyme) and counterstained with DAPI. Scale bars, 100 μm.

(C) Sections from day 35 HIOs were immunostained with antibodies to CDH17, CDX2, and PDX1. Sections were counterstained with DAPI. Scale bars, 10 μm.

(D) Sections from day 35 HIOs were immunostained with antibodies to DPP4, MUC2, and CHGA (inset, green). Immunofluorescence staining of day 35 spont- and agg-HIOs. Scale bar, 100 μm.