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Generation of HIOs from cryopreserved agg-spheroids
H1-derived spont-, agg-, and cryoagg-HIOs demonstrate similar expression of HIO differentiation markers DPP4, MUC2, and CHGA. Sections of day 35 spont-, agg-, and cryoagg HIOs were immunostained with antibodies to CDH1, DPP4, MUC2, and CHGA and counterstained with DAPI. Top and center panels scale bars, 100 μM. Bottom panel scale bar, 20 μm.
