Table 3.

Characterization of the processes expressing GFP^Astro in the AL. Fig. 5. shows representative images. The GFP^Astro area% increased after treatment with TrypLE (n = 8, p = 0.006). Wilcoxon signed rank tests compared values before to after treatment and Mann Whitney U tests compared values after buffer to those after TrypLE (n = 8 per group), p<0.05 is significant. Values are mean ± std.

	Group	Before	After	p-value Before vs. After	p-value After Buffer vs. After TrypLE
Area occupied by GFPAstro	Buffer	0.013 ± 0.003	0.012 ± 0.002	0.07	0.49
processes (mm2)	TrypLE	0.011 ± 0.001	0.011 ± 0.001	0.39
Total AL area (mm2)	Buffer	0.033 ± 0.007	0.031 ± 0.006	0.03	0.15
	TrypLE	0.031 ± 0.003	0.027 ± 0.002	0.0001
GFPAstro area%	Buffer	38.8% ± 4.1%	39.4% ± 5.0%	0.77	0.55
	TrypLE	37.2% ± 4.3%	41.5% ± 3.3%	0.0006
GFPAstro process width (μm)	Buffer	2.56 ± 0.10	2.57 ± 0.10	0.85	0.52
	TrypLE	2.57 ± 0.03	2.53 ± 0.06	0.25