Fig. 10.

LPC augmented hypoxia-elicited adenosine release but decreased extracellular and intracellular ATP produced by hypoxia. To mimic hypoxic conditions, BMDMs were cultured and treated as described before. Supernatants were collected to measure a extracellular adenosine, b eATP, and c iATP. Normoxic or hypoxic cell lysates (cytosolic and nuclear fraction) were harvested and analyzed by western blotting for d β-actin, HIF-1α expression, or e phospho-Cx43, total Cx43 proteins quantification, and results were expressed relative to that in resting normoxic state cells or β-actin content of the sample. Results are derived from 3 different experiments (mean ± SEM, n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001