Figure 1.

Spatial transcriptome analysis of mouse aging ovary

(A) Spatial transcriptome profiling workflow depicting collection, tissue handling, library preparation of two sections per capture area (4 capture areas and eight sections total), sequencing, and analysis.

(B and C) Unbiased identification of cell-type heterogeneity and biological variance in young versus aged mouse ovaries. (B) UMAP clustering is colored by identified cell clusters, (C) and sample group (young or aged).

(D) Cluster abundances by individual mouse ovary samples.

(E) Differences of cluster abundance plotted as log10 odds ratio against adjusted p value –log10 FDR. The solid horizontal line represents the significance threshold of 0.05 and the vertical dotted lines represent a 1.5-fold change in either direction.