Figure 6.

Cell-type specific gene expression changes reveal dysregulated pathways in aging ovaries

Ingenuity Pathway Analysis performed on all cluster DEG lists demonstrated a shared functional aging signature among cell clusters. (corpus luteum-regressing, CL-R; corpus luteum progressing, CL-P). EIF2 signaling, regulation of eIF4 and p70S6K signaling, mTOR signaling, and sirtuin signaling were all significant (FDR ≤0.05) in all clusters. Corpus luteum-R, stroma, and epithelium 1 share several other significant pathways including estrogen receptor signaling, oxidative phosphorylation and mitochondrial dysfunction, remodeling of epithelial adherens junctions, NRF2-mediated oxidative stress response, and BAG2 signaling.