Figure 7.

Analysis of sub-cluster heterogeneity in aged ovaries

UMAP clustering of sub-populations, colored by sub-cluster (left) and age group (middle). Differences in cluster abundance are plotted as log10 odds ratio against adjusted p value –log10 FDR (right).

(A) All corpus luteum-regressing (CL-R) sub-populations are significantly different in terms of relative proportion between young and aged ovary.

(B) There are four sub-populations within the corpus luteum-progressing (CL-P) cluster, of which sub-cluster 3 is significantly more abundant in aged ovaries.

(C) Stroma consists of eight sub-populations, six of which are significantly different in their relative abundance in aged compared to young.

(D) Follicles were found to have seven transcriptionally unique sub-populations; relative proportions of sub-clusters 0, 2, 5, and 6 were significantly different between young and aged ovaries.