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. 2022 Jul 27;6(3):txac097. doi: 10.1093/tas/txac097

Table 5.

Acetate, propionate, and butyrate molar proportion of total short-chain fatty acids concentrations (mM) produced by in vitro mixed ruminal microorganism fermentations containing 0 or 1.97 mM of camphor (CNT or CAM, respectively) and incubated for 0, 1, 2, 4, 12, or 24 h

Item Treatment P
LJC0 LJC30 HJC0 HJC30 SEM B×D MO Hour B D
CNT CAM CNT CAM CNT CAM CNT CAM
Acetate, mmol/100 mmol 0.34 <0.001 0.02 <0.001 0.01 <0.001
 0 H 74.46 76.38 76.19 76.86
 1 H 74.12 74.52 76.80 76.86 76.22 76.42 82.82 83.45
 2 H 79.32 79.41 82.40 84.27 76.93 76.86 77.04 77.02
 4 H 74.17 71.90 85.67 80.43 75.99 76.55 77.44 78.56
 12 H 74.41 81.21 85.12 80.85 77.49 77.42 79.66 79.68
 24 H 75.21 80.75 84.73 81.96 77.12 75.86 77.45 78.30
Propionate, mmol/100 mmol 0.24 0.13 0.01 <0.001 <0.001 <0.001
 0 H 17.20 15.77 17.05 15.15
 1 H 17.47 17.12 15.62 15.63 17.07 16.95 11.36 10.91
 2 H 14.11 14.06 12.05 10.74 16.65 16.69 15.32 15.32
 4 H 17.83 19.48 9.93 13.49 17.36 16.87 15.28 14.45
 12 H 18.10 13.16 10.61 13.62 16.56 16.58 14.19 14.13
 24 H 17.56 13.47 10.83 12.86 16.88 17.58 15.92 15.17
Butyrate, mmol/100 mmol 0.09 <0.001 0.02 <0.001 0.47 0.14
 0 H 7.19 7.09 5.82 7.40
 1 H 7.18 7.06 6.78 6.82 5.81 5.74 5.38 5.21
 2 H 5.71 5.69 5.06 4.55 5.54 5.57 7.06 7.08
 4 H 6.95 7.46 4.01 5.52 5.75 5.67 6.72 6.44
 12 H 6.52 4.89 3.89 5.04 5.16 5.22 5.70 5.71
 24 H 6.29 5.01 4.04 4.71 5.23 5.69 6.15 6.07

B × D, bloodline diet interaction across time and MO combinations; MO, monoterpene effect, control versus camphor nested within B × D group; hour, time effect, all incubation time points nested within B × D group; B, bloodline effect, LJC versus HJC; D, diet effect, 0 versus 30.