Table 5.
Acetate, propionate, and butyrate molar proportion of total short-chain fatty acids concentrations (mM) produced by in vitro mixed ruminal microorganism fermentations containing 0 or 1.97 mM of camphor (CNT or CAM, respectively) and incubated for 0, 1, 2, 4, 12, or 24 h
| Item | Treatment | P | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| LJC0 | LJC30 | HJC0 | HJC30 | SEM | B×D | MO | Hour | B | D | |||||
| CNT | CAM | CNT | CAM | CNT | CAM | CNT | CAM | |||||||
| Acetate, mmol/100 mmol | 0.34 | <0.001 | 0.02 | <0.001 | 0.01 | <0.001 | ||||||||
| 0 H | 74.46 | 76.38 | 76.19 | 76.86 | ||||||||||
| 1 H | 74.12 | 74.52 | 76.80 | 76.86 | 76.22 | 76.42 | 82.82 | 83.45 | ||||||
| 2 H | 79.32 | 79.41 | 82.40 | 84.27 | 76.93 | 76.86 | 77.04 | 77.02 | ||||||
| 4 H | 74.17 | 71.90 | 85.67 | 80.43 | 75.99 | 76.55 | 77.44 | 78.56 | ||||||
| 12 H | 74.41 | 81.21 | 85.12 | 80.85 | 77.49 | 77.42 | 79.66 | 79.68 | ||||||
| 24 H | 75.21 | 80.75 | 84.73 | 81.96 | 77.12 | 75.86 | 77.45 | 78.30 | ||||||
| Propionate, mmol/100 mmol | 0.24 | 0.13 | 0.01 | <0.001 | <0.001 | <0.001 | ||||||||
| 0 H | 17.20 | 15.77 | 17.05 | 15.15 | ||||||||||
| 1 H | 17.47 | 17.12 | 15.62 | 15.63 | 17.07 | 16.95 | 11.36 | 10.91 | ||||||
| 2 H | 14.11 | 14.06 | 12.05 | 10.74 | 16.65 | 16.69 | 15.32 | 15.32 | ||||||
| 4 H | 17.83 | 19.48 | 9.93 | 13.49 | 17.36 | 16.87 | 15.28 | 14.45 | ||||||
| 12 H | 18.10 | 13.16 | 10.61 | 13.62 | 16.56 | 16.58 | 14.19 | 14.13 | ||||||
| 24 H | 17.56 | 13.47 | 10.83 | 12.86 | 16.88 | 17.58 | 15.92 | 15.17 | ||||||
| Butyrate, mmol/100 mmol | 0.09 | <0.001 | 0.02 | <0.001 | 0.47 | 0.14 | ||||||||
| 0 H | 7.19 | 7.09 | 5.82 | 7.40 | ||||||||||
| 1 H | 7.18 | 7.06 | 6.78 | 6.82 | 5.81 | 5.74 | 5.38 | 5.21 | ||||||
| 2 H | 5.71 | 5.69 | 5.06 | 4.55 | 5.54 | 5.57 | 7.06 | 7.08 | ||||||
| 4 H | 6.95 | 7.46 | 4.01 | 5.52 | 5.75 | 5.67 | 6.72 | 6.44 | ||||||
| 12 H | 6.52 | 4.89 | 3.89 | 5.04 | 5.16 | 5.22 | 5.70 | 5.71 | ||||||
| 24 H | 6.29 | 5.01 | 4.04 | 4.71 | 5.23 | 5.69 | 6.15 | 6.07 | ||||||
B × D, bloodline diet interaction across time and MO combinations; MO, monoterpene effect, control versus camphor nested within B × D group; hour, time effect, all incubation time points nested within B × D group; B, bloodline effect, LJC versus HJC; D, diet effect, 0 versus 30.