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. 2019 Dec 13;24(1):13–24. doi: 10.1016/j.bjid.2019.11.003

Fig. 4.

Fig. 4

Standard curves of SYBR Green RT-qPCR for the titration of four dengue virus serotypes. Serial dilution of DENV was made before RNA extraction and amplification for each serotype. (A) Standard curve for DENV-1. Dilutions from 101 to 106 PFU/ml were made from the DENV-1 116 strain. (B) Standard curves for DENV-2. Dilutions from 102 to 106 PFU/ml were made from the DENV-1 216 strain. (C) Standard curve for DENV-3. Dilutions from 101 to 105 PFU/ml were made from the DENV-1 S23 strain. (D) Standard curve for DENV-4. Dilutions from 103 to 106 PFU/ml were made from the DENV-4 416 strain. Mean values of cycle thresholds corresponding a to standard plaque forming units are plotted. Each correlation coefficient and regression equation with 95% CI are shown.