Fig. 5.

Replicative fitness characteristics are determined by the serotype and the viral strain. Huh-7 cells were infected at an MOI 0.5 with any one of four dengue virus serotypes, and the cells were analyzed 72 h post-infection. (A) Replicative fitness quantification by qPCR. (B) Replicative fitness quantification by plaque assay. (C) Plaque assay with conditions of pH overlay medium and incubation days in a serotype-dependent manner (Crystal violet stain). Two independent experiments with three replicates where plotted. Horizontal lines indicate median IQR. *p < 0.05; **p < 0.005; ***p < 0.0005.