TABLE 1.
Bacteria and plasmids used in this study
| Strain or plasmid | Characteristic(s) | Source or reference |
|---|---|---|
| E. coli | ||
| DH5α | supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 λ− | Bethesda Research Laboratories |
| JM109 | endA1 recA1 gyrA96 hsdR17 supE44 λ− Δ(lac-proAB)/F′ [traD36 proAB+ lacZΔM15] | 68 |
| JM109 λ pir | JM109 lysogenized with λ pir bacteriophage | 47 |
| S17-1 | Tpr SmrhsdR pro recA RP4-2-Tc::Mu-Km::Tn7 in chromosome | 61 |
| S17-1 λ pir | S17-1 lysogenized with λ pir bacteriophage | 15 |
| XL1-Blue MR. | recA1 endA1 gyr-96 thi-1 hsdR17 supE44 relA1 lac/F′ [proAB+ lacZΔM15::Tn10 (Tcr)] | 11 |
| C600/pDPT51 | Mobilizing vector | 63 |
| R. capsulatus | ||
| ATCC 11166 | Wild type | 49 |
| TB2 | SB1003 regAΔEcoRI-PflMI::Kmr | 7 |
| SD01 | SB1003 regBΔ640bp N-terminus::Kmr | 18 |
| R. sulfidophilum | ||
| W4 | Wild type | 27 |
| RESA1 | regAΔPinAI-PinAI::Kmr | This study |
| RESB20 | regB::Kmr | This study |
| R. denitrificans | ||
| OCh114 | Wild type | 57 |
| Plasmids | ||
| SuperCos 1 | Apr; cosmid vector | Stratagene |
| pUC118 | Apr; cloning vector | 68 |
| pJP5603 | Kmr; R6K-based suicide vector | 47 |
| pSM3065 | Tcr; pJP5603 derivative | This study |
| pUC7Tc | Apr Tcr; source of the Tcr gene | 33 |
| pUCKM1 | Apr Kmr; source of the Kmr gene | 53 |
| pCB532Ω | Apr Smr Spr; translational fusion of pufQ to lacZ | 4 |
| pJRD215 | Kmr Smr; broad-host-range cosmid vector | 14 |
| pSLA | Apr; 5.0-kb EcoRI fragment (R. sulfidophilum regB-senC-regA) in pUC118 | This study |
| pROA | Apr; 5.5-kb EcoRI fragment (R. denitrificans regB-senC-regA) in pUC118 | This study |
| pSLKm4 | Apr Kmr; 6.3-kb fragment (R. sulfidophilum regAΔPinAI-PinAI::Kmr) in pUC118 | This study |
| pSA10 | Apr Kmr Tcr; 9.4-kb fragment (pSLKm4 digested with KpnI) in pSM3065 | This study |
| pSB07 | Kmr; 0.4-kb SacI-SalI fragment (part of R. sulfidophilum regB) in pJP5603 | This study |
| pMWS3.1 | Kmr Smr; 12-kb fragment (R. capsulatus senC-regA) in pJRD215 | 55 |
| pCSM9e | Kmr Smr; 5.1-kb fragment (R. capsulatus regB-senC) in pJRD215 | 42 |
| pMCS003 | Kmr Smr; 2.8-kb EcoRI-NruI fragment (R. sulfidophilum senC-regA) in pJRD215 | This study |
| pMCS010 | Kmr Smr; 2.6-kb EcoRI-NdeI fragment (R. sulfidophilum regB) in pJRD215 | This study |
| pMCR001 | Kmr Smr; 3.2-kb EcoRI-SacI fragment (R. denitrificans senC-regA) in pJRD215 | This study |
| pMCR010 | Kmr Smr; 2.5-kb HincII fragment (R. denitrificans regB-senC) in pJRD215 | This study |