TABLE 2.
Photopigment synthesis and photosynthetic gene expression in reg mutants of Rhodobacter capsulatus complemented with reg genes from various species
| Strain | BChl content (nmol/mg)c
|
Expression of puf operond
|
||
|---|---|---|---|---|
| −O2 | +O2 | −O2 | +O2 | |
| Wild type (R. capsulatus) | 34.5 | 0.5 | 544 ± 30 | 15 ± 5 |
| TB2(pJRD215)a vector only | 13.9 | 0.3 | 40 ± 10 | 9 ± 5 |
| TB2(pMWS3.1) +Rhodobacter regA-senC | 37.2 | 0.7 | 382 ± 50 | 62 ± 20 |
| TB2(pMCS003) +Rhodovulum regA-senC | 51.5 | 0.6 | 1,971 ± 150 | 125 ± 25 |
| TB2(pMCR001) +Roseobacter regA-senC | 42.5 | 0.6 | 315 ± 30 | 60 ± 15 |
| SD01(pJRD215)b vector only | 21.6 | 0.5 | 135 ± 70 | 18 ± 10 |
| SD01(pCSM9e) +Rhodobacter regB-senC | 42.8 | 0.5 | 1,050 ± 145 | 108 ± 40 |
| SD01(pMCS010) +Rhodovulum regB | 45.7 | 0.5 | 855 ± 150 | 60 ± 20 |
| SD01(pMCR010) +Roseobacter regB-senC | 40.1 | 0.4 | 499 ± 125 | 63 ± 25 |
a
TB2; R. capsulatus (ΔregA).
b
SD01; R. capsulatus (ΔregB).
c
BChl content in membranes (nanomoles per milligram of membrane protein). Data are based on the average of three independent assays. Uncertainty limits in this assay are within 5% in all transconjugants and the wild type. −O2, anaerobic photosynthetic growth; +O2, aerobic-dark growth.
d
Values are β-galactosidase activity (micromoles of o-nitrophenol-β-d-galactoside hydrolyzed per minute per milligram of protein) of strains with the puf::lacZ translational fusion in pCB532Ω (4).