Table 1.
Primers and standard curve parameters for hypertrophic marker amplification in C57Bl/6 mice infected with Trypanosoma cruzi.
| Mouse gene target | Primer sequences | Reference | Amplicon length | Slope | Intercept | Coefficient of linearity (r2) | Amplification efficiency (%) |
|---|---|---|---|---|---|---|---|
| GAPDH | Fw 5'-GGT TGC CAA ACC TTA TCA GAA ATG-3' | Ribeiro-Romão et al. [50] | 169 bp | −3.34 | 11.71 | 0.99 | 99.3 |
| Rv 5'-TTC ACC TGT TCC ACA GCC TTG-3' | |||||||
| HPRT | Fw 5' TCCCAGCGTCGTGATTAGCGATG-3' | Barreto-Albuquerque [51] | 178 bp | −3.44 | 21.15 | 0.98 | 95.3 |
| Rv 5'-GGCCACAATGTGATGGCCTCCC-3' | |||||||
| ANP | Fw 5'-GGATTTCAAGAACCTGCTAGACC-3' | This study | 167 bp | −3.27 | 19.69 | 0.99 | 102.1 |
| Rv 5′-CGTCTCTCAGAGGTGGGTTG-3 |