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. 2022 Aug 20;13:427. doi: 10.1186/s13287-022-03109-2

Table 1.

Gene-specific primers used in the real-time quantitative PCR

Gene Primer sequences (5′-3′)
Beclin-1

Forward 5′-TCATGCGATGGTGGCTTTCC-3

Reverse 5′-ATGGAATAGGAGCCGCCACT-3′

ATG5

Forward 5′-ACCTTTGCAGTGGCTGAGTG-3′

Reverse 5′-TCAATCTGTTGGTTGCGGGA-3′

ATG12

Forward 5′-CAACTGCTGCTGAGGGCGATG-3′

Reverse 5′-CACCGGCAGGTTCTTCTGTTCC-3′

P62 Forward 5′-CTGATGAAGGTGGCTGGCTGAC-3′Reverse 5′-CAAGGGCGGTGGGTGTTTCG-3′
PI3K

Forward 5′-ACGGAGGAGGTGCTCTGGAAC-3′

Reverse 5′-GGACTCGGGACTGGGCATCTC-3′

Akt

Forward 5′-GACGGCACCTTCATCGGCTAC-3′

Reverse 5′-CGCCACGGAGAAGTTGTTGAGG-3′

mTOR

Forward 5′-GCACGTCAGCACCATCAACCTC-3′

Reverse 5′-GCCTCAGCCATTCCAACCAGTC-3′