Fig. 1.

Dot1L is upregulated in TGF-β1-induced adult rat CFs and fibrotic mice hearts. A Dot1L, Col 1, FN, and CTGF protein expression level in cardiac fibroblasts (CFs) stimulated by different doses of TGF-β1 (0, 2, and 5 ng/mL) for 48 h. B Col 1, CTGF, Col 3, FN, and Dot1L protein expression level in cardiac fibroblasts stimulated by TGF-β1 (5 ng/mL) for the dedicated time (0, 12, 24, and 48 h). The data are presented as mean ± SD. Student’s t-test, ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001 versus control group, each acquired from three individual experiments. C Representative images of immunofluorescence staining of Dot1L as well as CTGF in CFs stimulated by 5 ng/mL TGF-β1 for 48 h. D Dot1L, Col 3 and CTGF protein expression around infarct area were measured in mice undergoing 2 weeks of left descending anterior coronary artery (LAD) ligation. The data are presented as mean ± SD. Student’s t-test, ^***p < 0.001 versus Sham group, n = 6/group