Fig. 5.

IR-iPSCs exhibit abnormal lactate secretion and are highly stimulated by insulin. A Quantitative PCR analysis for the lactate exporter gene SLC16A3. B Fold change in l-lactate secreted by IS- and IR-iPSCs in 15 min in KREB’s buffer. L-lactate secreted per ng of total protein for each IR-iPSC was normalized to average of that for IS-iPSCs in each experiment, C, D Western blot image for phosphorylation of AKT at Ser473 upon 0 nM or 100 nM insulin treatment in IS- and IR-iPSCs and its quantification performed using ImageJ software. *P value < 0.05, n = 2 clones