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Effect of peptide and ATP on the self-association of Hsp70Tm. (A) Untreated Hsp70Tm (1.5 μM). (B) Hsp70Tm (1.5 μM) incubated with peptide A (150 μM) at 70°C for 30 min. (C) Hsp70Tm incubated with peptide A (150 μM) and ATP (1 mM) at 70°C for 30 min. Peaks correspond to monomeric, dimeric, and multimeric Hsp70Tm, as labeled in panel A.
