FIG. 9.
sHspTm inhibition of GFP refolding at 55°C. (A) No added sHspTm; (B) 2:1 molar ratio of sHspTm to GFP; (C) 8:1 molar ratio of sHspTm to GFP; (D) 16:1 molar ratio of sHspTm to GFP; (E) 32:1 molar ratio of sHspTm to GFP. GFP denatured in 6 M guanidine HCl was diluted 1:520 into a stirred-fluorescence cuvette equilibrated at 55°C to a final concentration of 675 nM, with and without sHspTm. Excitation was at 488 nm, and GFP fluorescence was monitored at 512 nm.