FIG. 2.

AphB influences TcpA production in both classical and El Tor biotypes. Samples were prepared from KSK218 (classical [lane 1]), KSK218 with pKAS117 (AphB [lane 2]), GK122 (classical ΔaphB [lane 3]), GK122 with pKAS117 (AphB [lane 4]), KSK262 (El Tor [lane 5]), and KSK262 with pKAS117 (AphB [lane 6]). Cultures were grown overnight in LB medium (pH 6.5) at 30°C. Those with pKAS117 also contained 1 mM isopropyl-β-d-thiogalactopyranoside. Samples were analyzed by Western blotting with anti-TCP antiserum (34). TcpA is indicated by the arrow to the right.