TABLE 2.
Activation of a classical biotype tcpP-lacZ fusion by AphB and AphA
| Straina | β-Galactosidase activityb at pH and temp:
|
|||
|---|---|---|---|---|
| 6.5, 30°C | 8.5, 30°C | 6.5, 37°C | 8.5, 37°C | |
| KSK618 (tcpP-lacZ) | 3,940 ± 24 | 931 ± 8 | 2,091 ± 31 | 502 ± 11 |
| GK121 (ΔaphB) | 190 ± 22 | 145 ± 3 | 133 ± 8 | 98 ± 15 |
| KSK647 (ΔaphA) | 350 ± 75 | 104 ± 4 | 201 ± 6 | 85 ± 13 |
| KSK805 (ΔaphA ΔaphB) | 117 ± 7 | 97 ± 1 | 94 ± 7 | 74 ± 9 |
| GK121(pKAS117) (AphB) | 6,271 ± 680 | 5,428 ± 968 | 3,415 ± 74 | 2,616 ± 139 |
| KSK647(pKAS117) (AphB) | 3,283 ± 201 | 3,076 ± 408 | 1,669 ± 110 | 1,022 ± 61 |
| GK121(pKAS107) (AphA) | 502 ± 30 | 382 ± 88 | 288 ± 7 | 249 ± 1 |
| KSK647(pKAS107) (AphA) | 5,732 ± 723 | 2,589 ± 142 | 3,660 ± 846 | 1,816 ± 214 |
a
All plasmids were induced with 1 mM isopropyl-β-d-thiogalactopyranoside.
b
Units per optical density at 600 nm of culture.