TABLE 3.
Activation of an El Tor biotype tcpP-lacZ fusion by AphB and AphA
| Straina | β-Galactosidase activityb at pH and temp:
|
||||
|---|---|---|---|---|---|
| AKI medium | LB medium
|
||||
| 6.5, 30°C | 8.5, 30°C | 6.5, 37°C | 8.5, 37°C | ||
| KSK725 (tcpP-lacZ) | 1,060 ± 27 | 362 ± 8 | 172 ± 0 | 199 ± 8 | 101 ± 2 |
| GK138 (ΔaphB) | 129 ± 8 | 222 ± 6 | 162 ± 8 | 122 ± 8 | 94 ± 1 |
| GK161 (ΔaphA) | 122 ± 3 | 148 ± 1 | 104 ± 1 | 90 ± 3 | 70 ± 1 |
| KSK725(pKAS117) (AphB) | 2,129 ± 37 | 3,785 ± 309 | 1,270 ± 173 | 2,053 ± 168 | 753 ± 197 |
| KSK725(pKAS107) (AphA) | 1,060 ± 26 | 1,689 ± 17 | 730 ± 21 | 621 ± 41 | 284 ± 11 |
a
All plasmids were induced with 1 mM isopropyl-β-d-thiogalactopyranoside.
b
Units per optical density at 600 nm of culture.