Table 3.

Cost-effectiveness comparison of 20 DNA polymerases.

DNA polymerases	Item no.	Price (USD)	Tests	Price/test (USD)	Positive rate (n/12) (%)	Scores∗	Scores (price/test)
NEB Hemo klen Taq	M0332S	186.55	200	0.93	100.00	444	476.00
TAKARA MightyAmp	R071Q	40.95	40	1.02	100.00	444	433.69
Ultra HiFidelity PCR kit	KP203	97.28	80	1.22	100.00	444	365.14
TOYOBO KOD FX Neo	KFX-201S	31.38	20	1.57	100.00	444	282.98
Sangon Direct PCR kit	B639289-0050	82.37	50	1.65	100.00	444	269.51
TAKARA Tks Gflex	R060Q	75.47	80	0.94	100.00	344	364.65
TAKARA Taq	R001 A	18.83	200	0.09	83.30	244	2,591.88
Beyotime HemoTaq	D7241S	39.70	200	0.20	83.30	244	1,229.35
Mouse Tissue Direct PCR kit	KG205	62.76	50	1.26	83.30	244	194.39
NEB Q5	M0491S	137.92	100	1.38	83.30	244	176.92
TOYOBO KOD Plus Neo	KOD-401S	23.54	20	1.18	75.00	144	122.37
Phire Hot Start II	F122S	220.76	200	1.10	58.30	34	30.80
Platinum Direct PCR kit	A44647100	136.97	100	1.37	58.30	34	24.82
Beyotime Beyotime Taq	D7205	4.39	160	0.03	50.00	24	874.08
TOYOBO rTaq	TAP-211	23.54	100	0.24	41.70	14	59.49
Beyotime HemoTaq HF	D7243S	47.85	200	0.24	8.30	1	4.18
Beyotime BeyoFusion	D7220	16.95	80	0.21	0.00	0	0.00
Sangon Taq Plus	B500013-0100	3.45	20	0.17	0.00	0	0.00
StarLighter Blood Direct PCR	FS-P6001	18.83	500	0.04	0.00	0	0.00
NEB Vent	M0254 V	61.03	200	0.31	0.00	0	0.00

^∗ Scores were calculated based on the PCR results of 20 polymerases: 1 point for positive of 10³ parasites, 10 points for 10² parasites, and 100 points for 10¹ parasites.