FIG. 5.

α-MPP mRNA levels during B. emersonii development. Total RNA (10 μg/lane) isolated from cells at different stages of the B. emersonii life cycle were subjected to electrophoresis in a formaldehyde-agarose gel, transferred to Hybond N+ membrane, and probed with ³²P-labeled PCR-amplified fragment. Lanes: 1 to 5, sporulating cells 0, 60, 90, 120, and 180 min after starvation, respectively; 6, zoospores; 7 and 8, germinating cells 45 and 90 min after inoculation in DM3 medium. As a control, the same blot was hybridized to a ³²P-labeled rat GAPDH cDNA. (A) Autoradiograms of the blot. (B) Relative amount of α-MPP mRNA, determined by scanning of the autoradiograms, normalized with respect to the GAPDH signal.