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. 2022 Aug 20;5:848. doi: 10.1038/s42003-022-03781-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Plantlets of Salvia dominica grown in vitro. b Sterile leaf sections infected by A. rhizogenes. c Hairy roots (HRs) development from infected leaves. d Subcultures of excised hairy roots in solid hormone free medium. e HR in the hormone-free liquid MS medium. f Stable integration of the rolB gene in HRs assessed by polymerase chain reaction (PCR) in three independent transformation events. Amplicon length: 451 base pairs (bp) and 142 bp for rolB and actin genes, respectively. NTC, no template control. g Workflow planned for the purification and characterization steps of extracellular vesicles released by S. dominica HRs.