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. 2022 Aug 20;5:848. doi: 10.1038/s42003-022-03781-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a EVs size distribution curve obtained by dynamic light scattering (DLS). b Nanoparticle Tracking Analysis (NTA) measurements show the distribution of the EV size with the major peak at 151 nm. c TEM image of isolated vesicles exhibiting round-shaped morphology. d, e Close-up view images of individual HR-derived EVs. f Protein pattern of HR-derived EVs and EV depleted-medium (EDM) visualized by SDS-PAGE and silver staining. Silver staining of EDM did not show detectable proteins. Scale bars: 500 nm in c, 50 nm in d, 50 nm in e.