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. 2022 Aug 20;13:4913. doi: 10.1038/s41467-022-32649-7

Fig. 2. POU5F1B enhances CRC progression in vitro and in vivo.

Fig. 2

a Western blot analysis of HA-tagged GFP and POU5F1B in SW480 cells (representative blot out of three independent experiments). b Colony formation assay in GFP- and POU5F1B-overexpressing SW480 cells (n = 3 independent experiments; P = 0.011 by two-sided t-test). c 3-(4,5-dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide (MTT) assay in GFP- and POU5F1B-overexpressing SW480 cells (n = 3 independent experiments; P = 4.67e-05 by two-sided Wilcoxon test). d Tumor volume and weight in subcutaneous implanted POU5F1B-overexpressing SW480 cells (n = 7 tumors per group; P = 0.041 by two-sided Wilcoxon test; P = 0.048 by two-sided t-test, respectively), with representative macroscopy. e Tumor invasion based on bioluminescence in orthotopically implanted animals (n = 10 animals per group, P = 0.038 by two-sided Wilcoxon test). f Number of macrometastases in liver left lobe and of additional organs affected by metastases (n = 10 animals per group, P = 0.002, P = 0.009, respectively, by two-sided t-test). g Number of macrometastases in liver left lobe following intrasplenic injection and splenectomy, with at right macroscopic view and microscopy of H&E-stained slices (n = 7 animals per group, P = 2.4e–04 by two-sided t-test). h MTT assay (n = 3 independent experiments; sh3 P = 2e–04, sh5 P = 1.19e–06 by Wilcoxon test) of LS1034 cells transduced with two different TcGT-targeting shRNAs or a control scramble sequence. i Tumor volume and weight in mice subcutaneously implanted with POU5F1B-downregulated or control LS1034 cells (n = 16 tumors scramble and n = 8 tumors shRNA, P = 0.018, P = 1e–04 respectively, by two-sided Wilcoxon test, boxplots left panel are represented as first and third quartiles with median in the center and whiskers are defined as 1.5 times the interquartile range clipped to the maximum/minimum of the data), with representative macroscopy (shRNA3 left, shRNA5 right). Data in b–h, i right) are presented as mean values ± s.e.m., with single values as circles. *P < 0.05, **P < 0.01, ***P < 0.001. Source data are provided as a Source Data file.