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Independent variables and response factors opted for optimization of IMQ-nTES.
| Independent variables | Levels analyzed | |
|---|---|---|
| Low level (− 1) | High level (+ 1) | |
| X1: PL90G:EA conc. (mg) | 75:25 | 95:5 |
| X2: ethanol conc. (% v/v) | 20 | 40 |
| X3: stirring rate (rpm) | 700 | 1200 |
| Response factors (dependent) | Desirable response | |
|---|---|---|
| Y1: vesicle size (nm) | Minimize | |
| Y2: poly-dispersibility | Minimize | |
| Y3: entrapment efficiency (%) | Maximize |
