Table 7.

ATR-FTIR skin analysis for determination of skin structure alteration.

Group	ATR-FTIR band position (cm−1)	ATR-FTIR Band Intensity	Bond stretching	Interpretation by ATR-FTIR band position and intensity
Control group	3290	0.36	–OH and –NH stretching	Normal protein and water content in skin
	2965	0.10	–CH (methyl and methylene) stretching of lipids and proteins	Normal protein and lipid content in skin
	2877	0.07
	1690	0.30	C–N stretching, α-helix, and random coiling of proteins	The secondary structure of cutaneous proteins was preserved
	1610	0.18
Blank TES gel	3290	0.36	–OH and –NH stretching	Normal protein and water content in skin
	2968	0.12	–CH (methyl and methylene) stretching of lipids and proteins	Normal protein and lipid content in skin
	2878	0.08
	1695	0.29	C–N stretching, α-helix, and random coiling of proteins	The secondary structure of cutaneous proteins was preserved
	1620	0.17
ALDARA	3310	0.45	–OH and –NH stretching	Higher band intensity was due to increment in water, protein, and glycosaminoglycans content in the skin
	2970	0.16	–CH (methyl and methylene) stretching of lipids and proteins	Increased intensity in –CH (methyl and methylene) stretching predicting the increased protein and lipid content in skin
	2870	0.10
	1650	0.53	C–N stretching, α-helix, and random coiling of proteins	Alteration in the secondary structure of cutaneous proteins due to the band shifting with enhanced proteinaceous content
	1580	0.38
IMQ-nTES gel	3306	0.33	–OH and –NH stretching	A slight elevation in cutaneous protein and water content
	2970	0.13	–CH (methyl and methylene) stretching of lipids and proteins	A slight increment in lipid content in skin
	2869	0.09
	1699	0.33	C–N stretching, α-helix, and random coiling of proteins	The secondary structure of cutaneous proteins was not altered, however, a slight increment in protein content was observed
	1625	0.23